Tissue culture or tissue culture is a maintenance technique or part of the individual networks in artificial (artificial). The definition of artificial is done outside the individual. Therefore, this technique is often called the culture in vitro, as opposed to in vivo. Told in vitro (Latin, meaning "in glass") because the tissue cultured in the incubation tube or Petri dish of glass or other opaque material. Tissue culture can theoretically be done for all networks, both from plants and animals (including humans), but each network requires a specific media composition.
Implementation of these techniques require many prerequisites for supporting life of the cultured tissue. The most essential is a container and a sterile growing medium. Media is a place for networks to grow and take the nutrients that support life on the network. The growth medium provides a variety of materials necessary for living tissue and reproduce themselves.
There are two classification of growth media: the media in solid and liquid media. Solid media is generally a solid gel, such as agar. Nutrition mixed on agar. Liquid media, nutrients are dissolved in water. Liquid media can be either calm or in a state of constant motion, depending on needs.
Theoretical basis of in vitro culture is this Totipotensi.Teori believe that every part of the plant can grow .because all parts of the plant consists of a network - a network of life.
Is the technique of tissue culture propagation of plants by way of isolating the parts of plants such as leaves, buds, and grow the parts made aseptically in media-rich nutrients and growth regulators in closed containers translucent so that the plants can reproduce themselves and generations to become complete plants. The main principle is the propagation of plants using vegetative parts of plants, using artificial media was conducted in a sterile place. Tissue culture techniques at this time has developed into plant breeding techniques are very important in various plant species.
B. BASIC PRINCIPLES OF CULTURE NETWORKS
The basic principle of tissue culture
a. Cells of a multicellular organism wherever located, are virtually identical to cells derived from a single zygote because these cells (Every cell comes from a single cell).
b. Theory Totipotensi Cell (Total Genetic Potential), meaning that every cell has the genetic potential of such zygotes are able to reproduce themselves and berediferensiasi into complete plants. This theory believes that every part of the plant can grow biak.karena all parts of the plant consists of a network - a network of life.
Implementation of these techniques require many prerequisites for supporting life of the cultured tissue. The most essential is a container and a sterile growing medium. Media is a place for networks to grow and take the nutrients that support life on the network. The growth medium provides a variety of materials necessary for living tissue and reproduce themselves.
There are two classification of growth media: the media in solid and liquid media. Solid media is generally a solid gel, such as agar. Nutrition mixed on agar. Liquid media, nutrients are dissolved in water. Liquid media can be either calm or in a state of constant motion, depending on needs.
Theoretical basis of in vitro culture is this Totipotensi.Teori believe that every part of the plant can grow .because all parts of the plant consists of a network - a network of life.
Is the technique of tissue culture propagation of plants by way of isolating the parts of plants such as leaves, buds, and grow the parts made aseptically in media-rich nutrients and growth regulators in closed containers translucent so that the plants can reproduce themselves and generations to become complete plants. The main principle is the propagation of plants using vegetative parts of plants, using artificial media was conducted in a sterile place. Tissue culture techniques at this time has developed into plant breeding techniques are very important in various plant species.
B. BASIC PRINCIPLES OF CULTURE NETWORKS
The basic principle of tissue culture
a. Cells of a multicellular organism wherever located, are virtually identical to cells derived from a single zygote because these cells (Every cell comes from a single cell).
b. Theory Totipotensi Cell (Total Genetic Potential), meaning that every cell has the genetic potential of such zygotes are able to reproduce themselves and berediferensiasi into complete plants. This theory believes that every part of the plant can grow biak.karena all parts of the plant consists of a network - a network of life.
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